Golgi-Apparatus

Golgi Apparatus / Complex [Structure, Functions, Products]

Golgi Apparatus / Golgi Complex

Golgi device was found in the year 1898 by an Italian biologist Camillo Golgi.

The Golgi apparatus or the Golgi body or Golgi complex or merely Golgi is a cellular organelle present in the majority of the cells of eukaryotic organisms. It is described as the manufacturing and shipping center of the cell.

Golgi is associated with the product packaging of the protein molecules before they are sent to their destination.

These organelles assist in processing and product packaging the macromolecules like proteins and lipids that are manufactured by the cell and thus function as the ‘post office’ of the cell.

The Golgi apparatus is located in between the endoplasmic reticulum and the cell membrane.

Most often, the Golgi appears to be an extension of the endoplasmic reticulum which is slightly smaller and smoother in appearance. However, the Golgi apparatus can be easily misinterpreted for smooth endoplasmic reticulum. Although they look comparable, the Golgi is an independent organelle that has different functions.

Structure of Golgi Apparatus

Under the electron microscope, the Golgi apparatus is seen to be composed of stacks of flattened structures that contain various vesicles including secretory granules. The Golgi complex is morphologically very comparable in both plant and animal cells.

However, it is exceptionally pleomorphic: in some cell types it appears compact and restricted, in others spread out and reticular (net-like). Typically, however, the Golgi complex looks like a complicated selection of interconnecting tubules, vesicles, and cisternae.

Golgi-Structure

A.Cisternae

The simplest unit of the Golgi complex is the cisterna. Cisternae (about 1 μm in size) are central, flattened, plate-like, or saucer-like closed compartments that are kept in parallel packages or stacks one above the other.In each stack, cisternae are separated by an area of 20 to 30 nm which might consist of rod-like elements or fibers.

Each stack of cisternae forms a dictyosome which might consist of 5 to 6 Golgi cisternae in animal cells or 20 or more cisternae in plant cells. Each cisterna is bounded by a smooth unit membrane (7.5 nm thick), having a lumen varying in width from about 500 to 1000 nm. The margins of each cisterna are gently curved so that the entire dictyosome of the Golgi apparatus takes on a bow-like appearance. The cisternae at the convex end of the dictyosome make up proximal, forming, or cis-face, and cisternae at the concave end of the dictyosome make up the distal, growing or trans-face.

B.Tubules

A complicated variety of associated vesicles and anastomosing tubules (30 to 50 nm diameter) surround the dictyosome and radiate from it. In fact, the peripheral area of the dictyosome is fenestrated (lace-like) in structure.

C.Vesicles

The vesicles (60 nm in size) are of three types:

  • (i) Transitional vesicles are small membrane-limited vesicles which are thought to form as blebs from the transitional ER to move and converge to the cis face of Golgi, where they coalesce to form new cisternae.
  • (ii) Secretory vesicles are varied-sized membrane-limited vesicles that release from margins of cisternae of Golgi. They, typically, present in between the growing or maturing face of Golgi and the plasma membrane.
  • (iii) Clathrin-coated vesicles are spherical protrusions, about 50 μm in size, and with a rough surface. They are present at the periphery of the organelle, typically at the ends of single tubules, and are morphologically rather distinct from the secretory vesicles. The Clathrin-coated vesicles are understood to play a role in intracellular traffic of membranes and of secretory items, i.e., in between ER and Golgi, along with, between the GELR area and the endosomal and lysosomal compartments.

Golgi-Vesicles

 

Golgi Apparatus Functions

The Golgi apparatus has lots of discrete functions. But all functions are related to moving molecules from the endoplasmic reticulum to their last location and customizing particular products along the way. The numerous sacs of the Golgi serve as different chambers for chemical reactions. As the products of the endoplasmic reticulum relocation through the Golgi apparatus, they are continuously transferred into new environments, and the reactions that can occur are different.

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In this way, an item can be given modifications, or multiple items can be combined to form big macromolecules. The many sacs and folds of the Golgi apparatus enable numerous reactions to take place at the same time, increasing the speed at which an organism can produce products.

Golgi-Functions

Tagging Cellular Products

Regardless of the item, the vesicles consisting of the product move from the endoplasmic reticulum and into the cis face of the Golgi apparatus. In layman’s terms, this is the side facing the endoplasmic reticulum. The side outermost from the endoplasmic reticulum is referred to as the trans face of the Golgi apparatus, and this is where products are headed.

After having any modifications or additions to their structure, the items are packaged in vesicles and tagged with markers that suggest where the vesicle needs to end up. These tags can be molecules, such as phosphate groups, or unique proteins on the surface of the vesicle. As soon as tagged, the vesicle is excreted from the Golgi complex, on its way to its final location.

Finalizing Cellular Products

In secretory cells, or cells that produce big amounts of a substance that your body needs, the Golgi complex will be large. Consider the cells in your stomach that produce acid. The acid is produced by reactions in the endoplasmic reticulum and is modified as it passed through the Golgi complex. When to the trans side of the Golgi apparatus, the acid is packaged in a vesicle and sent towards the cell’s surface. As the vesicle joins with the plasma membrane, the acid is released into the stomach, so it can absorb your food.

Golgi-Products

Golgi Complex in Plant Cells

While this article primarily discusses the operation of the Golgi apparatus within animal cells, plant cells also have a Golgi apparatus. In fact, plant cells may consist of hundreds of these organelles.

Within plant cells, the Golgi apparatus serves the additional function of synthesizing the major polysaccharide molecules which help in the formation of the cell wall. To do this, plants frequently have a lot more Golgi bodies than an animal cell.

Even more, plant cells do not include lysosomes. These digestive organelles are replaced in the plant with the central vacuole, which serves as a large lysosome along with an organelle to store water. Hence, lots of vesicles from the Golgi bodies of plants transfer to the vacuole and fuse their contents with this big organelle.

MCQs

  • Question 1: Who discovered the Golgi apparatus?
    • A. Robert Hooke
    • B. Anton van Leeuwenhoek
    • C. Camillo Golgi
    • D. Louis Pasteur
    • Answer: C
  • Question 2: What is another name for the Golgi apparatus?
    • A. Endoplasmic Reticulum
    • B. Ribosome
    • C. Golgi Complex
    • D. Nucleus
    • Answer: C
  • Question 3: Where is the Golgi apparatus located in the cell?
    • A. Next to the nucleus
    • B. Between the endoplasmic reticulum and the cell membrane
    • C. Inside the mitochondria
    • D. Attached to the cell wall
    • Answer: B
  • Question 4: What is the main function of the Golgi apparatus?
    • A. Energy production
    • B. Protein synthesis
    • C. Manufacturing and shipping of cellular products
    • D. DNA replication
    • Answer: C
  • Question 5: Which term is used to describe the stacks of flattened structures in the Golgi apparatus?
    • A. Cristae
    • B. Cisternae
    • C. Tubules
    • D. Vesicles
    • Answer: B
  • Question 6: What is the function of tubules in the Golgi apparatus?
    • A. Packaging cellular products
    • B. Transporting molecules to the endoplasmic reticulum
    • C. Surrounding the dictyosome
    • D. Facilitating DNA replication
    • Answer: C
  • Question 7: Which type of vesicles is thought to form from the transitional ER and move to the cis face of the Golgi?
    • A. Transitional vesicles
    • B. Secretory vesicles
    • C. Clathrin-coated vesicles
    • D. Golgi vesicles
    • Answer: A
  • Question 8: What is the term for the side of the Golgi apparatus facing the endoplasmic reticulum?
    • A. Proximal face
    • B. Distal face
    • C. Trans face
    • D. Cis face
    • Answer: D
  • Question 9: What is the primary role of the Golgi apparatus in secretory cells?
    • A. Energy production
    • B. Protein synthesis
    • C. Packaging and releasing cellular products
    • D. DNA replication
    • Answer: C
  • Question 10: Which term refers to the process of tagging vesicles with markers indicating their destination?
    • A. Vesicle transport
    • B. Tagging process
    • C. Exocytosis
    • D. Receptor-mediated endocytosis
    • Answer: B
  • Question 11: In which type of cells would you expect to find a large Golgi complex?
    • A. Nerve cells
    • B. Muscle cells
    • C. Secretory cells
    • D. Blood cells
    • Answer: C
  • Question 12: What happens to cellular products after modifications in the Golgi apparatus?
    • A. They are sent to lysosomes
    • B. They are packaged in vesicles and tagged for release
    • C. They are transported to the nucleus
    • D. They are converted into energy
    • Answer: B
  • Question 13: Which additional function does the Golgi apparatus serve in plant cells?
    • A. Synthesizing DNA
    • B. Energy production
    • C. Synthesizing cell wall polysaccharides
    • D. Protein synthesis
    • Answer: C
  • Question 14: What is the term for the large lysosome-like organelle in plant cells?
    • A. Golgi vesicle
    • B. Vacuole
    • C. Ribosome
    • D. Endosome
    • Answer: B
  • Question 15: Who is credited with the discovery of cisternae, the simplest unit of the Golgi complex?
    • A. Robert Hooke
    • B. Anton van Leeuwenhoek
    • C. Camillo Golgi
    • D. Louis Pasteur
    • Answer: C
  • Question 16: What is the primary function of clathrin-coated vesicles in the Golgi apparatus?
    • A. Secretion of cellular products
    • B. Intracellular traffic of membranes and secretory items
    • C. Synthesizing proteins
    • D. Packaging DNA
    • Answer: B
  • Question 17: Which organelle is often mistaken for the Golgi apparatus due to their similar appearance?
    • A. Mitochondria
    • B. Endoplasmic reticulum
    • C. Nucleus
    • D. Ribosome
    • Answer: B
  • Question 18: What is the role of transitional vesicles in the Golgi apparatus?
    • A. Forming new cisternae in the trans face
    • B. Synthesizing proteins
    • C. Packaging cellular products
    • D. Transporting molecules to the endoplasmic reticulum
    • Answer: A
  • Question 19: How is the Golgi apparatus described in terms of its appearance in various cell types?
    • A. Always compact and restricted
    • B. Always spread out and reticular
    • C. Morphologically very similar in all cell types
    • D. Highly pleomorphic, appearing differently in different cell types
    • Answer: D
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FAQs – Golgi Apparatus / Complex [Structure, Functions, Products]

1. What is the Golgi Apparatus, and who discovered it?

  • Answer: The Golgi apparatus, also known as the Golgi complex, is a cellular organelle responsible for packaging and modifying proteins and lipids. It was discovered by the Italian biologist Camillo Golgi in the year 1898.

2. Where is the Golgi Apparatus located in the cell?

  • Answer: The Golgi apparatus is located between the endoplasmic reticulum and the cell membrane.

3. How is the structure of the Golgi Apparatus described?

  • Answer: Under the electron microscope, the Golgi apparatus appears as stacks of flattened structures (cisternae) with associated vesicles and tubules. It is morphologically similar in both plant and animal cells but exhibits pleomorphism, appearing differently in various cell types.

4. What are Cisternae in the Golgi Apparatus?

  • Answer: Cisternae are the simplest units of the Golgi complex. They are central, flattened, plate-like compartments that are stacked in parallel packages. Each stack forms a dictyosome, and cisternae play a role in the processing and packaging of cellular products.

5. Explain the function of Tubules and Vesicles in the Golgi Apparatus.

  • Answer: Tubules and vesicles surround the dictyosome of the Golgi apparatus. Tubules are anastomosing structures that radiate from the dictyosome, and vesicles include transitional vesicles (involved in the formation of new cisternae), secretory vesicles, and clathrin-coated vesicles.

6. What are the primary functions of the Golgi Apparatus?

  • Answer: The Golgi apparatus is involved in moving molecules from the endoplasmic reticulum to their final destination and modifying specific products. It serves as chambers for various chemical reactions, allowing the modification and combination of molecules to form macromolecules.

7. How does the Golgi Apparatus tag cellular products?

  • Answer: Vesicles containing cellular products move from the endoplasmic reticulum to the cis face of the Golgi apparatus. The products are tagged with markers, such as phosphate groups or specific proteins, indicating their destination. Tagged vesicles are then excreted from the Golgi complex.

8. What is the significance of the Golgi Complex in plant cells?

  • Answer: Plant cells also have a Golgi apparatus, and it plays a crucial role in synthesizing major polysaccharide molecules that contribute to the formation of the cell wall. Plant cells may contain numerous Golgi bodies, and vesicles from these bodies often fuse with the central vacuole in plant cells.

9. Is the Golgi Apparatus present in all eukaryotic cells?

  • Answer: Yes, the Golgi apparatus is present in the majority of eukaryotic cells, contributing to the processing and packaging of macromolecules.

10. How does the Golgi Apparatus contribute to the functioning of secretory cells?

  • Answer: In secretory cells, the Golgi complex is large and plays a crucial role in processing and modifying substances produced by the cell. The modified products are then packaged in vesicles and released from the cell.
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11. Can the Golgi Apparatus be mistaken for the smooth endoplasmic reticulum?

  • Answer: Yes, the Golgi apparatus can be easily misinterpreted for the smooth endoplasmic reticulum due to their similar appearance. However, they are independent organelles with distinct functions.

 

Summary: Golgi Apparatus Tutorial

The Golgi Apparatus, discovered in 1898 by Camillo Golgi, stands as a vital cellular organelle in eukaryotic organisms. Positioned between the endoplasmic reticulum and the cell membrane, it acts as the cell’s manufacturing and shipping center, responsible for packaging proteins and lipids. While it may resemble an extension of the endoplasmic reticulum, the Golgi apparatus holds distinct functions.

Structure of Golgi Apparatus

Under the electron microscope, the Golgi apparatus reveals a complex structure:

  • Cisternae: Basic units forming dictyosomes, central, flattened, plate-like compartments in stacks. They play a pivotal role in cellular processing.
  • Tubules: Associated vesicles and anastomosing tubules surrounding the dictyosome contribute to its structural dynamics.
  • Vesicles: Three types, including transitional vesicles, secretory vesicles, and clathrin-coated vesicles, participate in various cellular processes.

Golgi Apparatus Functions

This cellular organelle serves as a multifaceted chamber for chemical reactions, allowing for the modification and combination of molecules during the transportation of products from the endoplasmic reticulum. This intricate process facilitates the customization of cellular products and the formation of large macromolecules.

Tagging Cellular Products

Products are tagged for their destination as vesicles move from the endoplasmic reticulum to the cis face of the Golgi apparatus. These tags, such as phosphate groups or specific proteins, guide the vesicles to their intended locations. Once tagged, the vesicles are released from the Golgi complex, finalizing the cellular products.

Finalizing Cellular Products

In secretory cells, particularly those producing substantial amounts of essential substances, the Golgi complex plays a crucial role. When large, it modifies and packages products for secretion, as seen in cells producing stomach acid.

Golgi Complex in Plant Cells

The Golgi apparatus is not exclusive to animal cells; plant cells also possess this organelle, often in abundance. In plants, it contributes to synthesizing polysaccharide molecules for cell wall formation. Unlike animal cells, plant cells lack lysosomes, and Golgi-derived vesicles often fuse with the central vacuole to facilitate storage and digestion.

In conclusion, the Golgi apparatus is a dynamic and indispensable component of cellular function, orchestrating the intricate processes of modification, packaging, and transportation of cellular products in both animal and plant cells.